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Acid Fast - Revision history
2026-08-25T11:26:07Z
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MedLabWIki>Admin at 17:52, 17 September 2023
2023-09-17T17:52:32Z
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<p><b>New page</b></p><div>Acid fast organisms have a thick cell wall that is waxy due to the presence of mycolic acids. As a result, these organisms resist decolourization by acid alcohol.<br />
{| class="wikitable"<br />
|+<br />
!<br />
!Ziehl-Neelsen (ZN) Stain<br />
!Kinyoun Stain<br />
|-<br />
|Primary Stain<br />
|Carbol fuchsin<br />
<br />
* '''Heated''' on slide<br />
|Carbol fuchsin<br />
<br />
* Uses '''higher phenol''' concentration at '''normal temperature'''<br />
|-<br />
|Decolourizer<br />
|3% acid alcohol*<br />
|3% acid alcohol*<br />
|-<br />
|Counterstain<br />
|Methylene blue<br />
|Methylene blue<br />
|-<br />
|<br />
|ZN (Zinc) hot<br />
|Kinyoun, cold, concentrated<br />
|}<br />
<nowiki>*</nowiki>Modified acid fast stains use a lower concentration of acid alcohol (0.5-1.0%) for organisms with less mycolic acid present in their cell walls.<br />
<br />
Acid Fast Organisms<br />
<br />
* Mycobacteria (e.g., M. tuberculosis, M. leprae, etc.)<br />
<br />
Partially Acid Fast Organisms<br />
<br />
* Nocardia<br />
* Legionella</div>
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