https://medlabwiki.com/index.php?action=history&feed=atom&title=Blood_Basics 2026-05-25T19:24:13Z Revision history for this page on the wiki MediaWiki 1.45.3 https://medlabwiki.com/index.php?title=Blood_Basics&diff=123&oldid=prev 2025-01-07T03:36:09Z

<p>Created page with &quot;Composition of Blood * ~45% red blood cells * ~55% plasma * &lt;1% leukocytes and thrombocytes Peripheral Blood Films * Anticoagulated whole blood, venous (EDTA lavender top) ** EDTA (ethylenediaminetetraacetic acid) chelates calcium to prevent activation of coagulation cascade ** Preserves cell components, minimal affects on cell morphology *** Excess EDTA (underfilled) can cause: **** Low cell counts &amp; hematocrit **** Altered staining and morphology ***** Cell shrinkag...&quot;</p> <p><b>New page</b></p><div>Composition of Blood<br /> <br /> * ~45% red blood cells<br /> * ~55% plasma<br /> * &lt;1% leukocytes and thrombocytes<br /> <br /> Peripheral Blood Films<br /> <br /> * Anticoagulated whole blood, venous (EDTA lavender top)<br /> ** EDTA (ethylenediaminetetraacetic acid) chelates calcium to prevent activation of coagulation cascade<br /> ** Preserves cell components, minimal affects on cell morphology<br /> *** Excess EDTA (underfilled) can cause:<br /> **** Low cell counts &amp; hematocrit<br /> **** Altered staining and morphology<br /> ***** Cell shrinkage, crenation, WBC damage<br /> *** Low EDTA (overfilled) can cause clotting and platelet clumps<br /> ** Store maximum 4-5 hours at RT before making films or running CBC (12 hours at 4C)<br /> *** Prolonged storage in EDTA can cause:<br /> **** Artifacts (crenated cells and spherocytes)<br /> **** Increased MCV and HCT<br /> **** Decreased cell counts, necrotic cells, vacuolation<br /> * Capillary collection before clotting<br /> * Making films<br /> ** Want a slightly rounded, feathered edge with good monolayer<br /> ** Spread with slide at 30-45 degree angle<br /> ** Larger WBCs may have tendency to be pushed to edge of smear (especially monocytes and granulocytes)<br /> * Slide Staining<br /> ** Romanowsky staining (Wright, Wright-Giemsa, etc.)<br /> *** Polychromatic stain<br /> *** Eosin and methylene blue, buffered at pH 6.4-6.8 for staining to occur<br /> **** Eosin: acidic (-), stains basic components red-orange<br /> ***** Hemoglobin, eosinophilic granules (protein amine groups)<br /> **** Methylene blue: basic (+), stains acidic components blue<br /> **** Thiazine-eosinate complex: neutral, stains neutral components mix of red and blue<br /> ***** Neutrophil granules (lilac coloured)<br /> ****** Nucleic acids, basophil granules (carboxyllic acids and phosphoric acid groups)<br /> *** If pH is too basic, more things will appear gray/blue. If pH is too acidic, more things will appear red-orange</div>

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