GI Specimen Processing - Revision history

Admin at 16:55, 1 April 2024

2024-04-01T16:55:28Z

@@ Line 13: / Line 13: @@
 ==== Spot Oxidase ====
-. put filter paper on microscope slide
+. Put filter paper on microscope slide
-. add drop of oxidase reagent to filter paper
+. Add a drop of oxidase reagent to the filter paper
-. Let dry at least 1 min (IMPORTANT: will get false neg otherwise)
+. Let dry for 1-2 minutes ('''IMPORTANT:''' must let dry for at least 1 minute, otherwise it may give a false negative result)
-. Get colony on applicator stick and press and hold onto paper for 5 sec
+. Get a colony on an applicator stick. Press and hold it onto the paper for 5 sec.
-. read results (positive blue)
+. Read results (positive: blue)
 ==== Other Notes ====
@@ Line 35: / Line 35: @@
 Check for purity from MAC plate.
 ==== Organisms ====

Admin: /* Order of inoculating GIT tubes */

2024-03-25T03:07:05Z

Order of inoculating GIT tubes

← Older revision		Revision as of 23:07, 24 March 2024
Line 1:		Line 1:
	==== Order ~~of inoculating~~ GIT tubes ====		==== Order for Inoculating GIT tubes ====
	1. MAC		1. MAC (small line)

	2. BA		2. BA (small line)

	3. TSI		3. TSI (down to 1/4" from bottom in straight line, then streak surface)

	4. ONPG PAM		4. ONPG-PAM (down to 1/4" from bottom in straight line)

	5. TSB (swish around)		5. TSB (swish around)

			Then return to MAC and BA to streak out the quadrants.

	==== Spot Oxidase ====		==== Spot Oxidase ====

Admin: Created page with "==== Order of inoculating GIT tubes ==== 1. MAC 2. BA 3. TSI 4. ONPG PAM 5. TSB (swish around) ==== Spot Oxidase ==== 1. put filter paper on microscope slide 2. add drop of oxidase reagent to filter paper 3. Let dry at least 1 min (IMPORTANT: will get false neg otherwise) 4. Get colony on applicator stick and press and hold onto paper for 5 sec 5. read results (positive blue) ==== Other Notes ==== Serology: perform from TSI or BApp (cannot use anything that's s..."

2024-03-25T03:05:59Z

Created page with "==== Order of inoculating GIT tubes ==== 1. MAC 2. BA 3. TSI 4. ONPG PAM 5. TSB (swish around) ==== Spot Oxidase ==== 1. put filter paper on microscope slide 2. add drop of oxidase reagent to filter paper 3. Let dry at least 1 min (IMPORTANT: will get false neg otherwise) 4. Get colony on applicator stick and press and hold onto paper for 5 sec 5. read results (positive blue) ==== Other Notes ==== Serology: perform from TSI or BApp (cannot use anything that's s..."

New page

==== Order of inoculating GIT tubes ====
1. MAC

2. BA

3. TSI

4. ONPG PAM

5. TSB (swish around)

==== Spot Oxidase ====
1. put filter paper on microscope slide

2. add drop of oxidase reagent to filter paper

3. Let dry at least 1 min (IMPORTANT: will get false neg otherwise)

4. Get colony on applicator stick and press and hold onto paper for 5 sec

5. read results (positive blue)

==== Other Notes ====
Serology: perform from TSI or BApp (cannot use anything that's selective)

* Serology may take a while to show agglutination - use a thick/milky suspension and rock card back and forth for 1-2 minutes (may need to wait the full 2 minutes)

Indole: use TSB tube (make sure you're using correct reagent - Kovacs!) or spot indole from BApp. If doing motility, take drop before adding reagent.

Motility darkfield: use TSB sample or colony from BApp with saline drop

ONPG: Check ONPG and motility first, then add ferric chloride.

Check for purity from MAC plate.

==== Organisms ====
very tiny clear things on plate - bile salts precipitating out (likely that your plate has been refrigerated)

Edwardsiella are always indole + but otherwise appear to be Salmonella (which is indole -)

Shigella may appear similar to Salmonella, but is non-motile.

Proteus is PPD + (organisms of interest are all PPD -)