Admin at 23:10, 20 February 2025

2025-02-20T23:10:24Z

← Older revision		Revision as of 19:10, 20 February 2025
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	*** Airborne		*** Airborne
			S

			* Sterilization: kills all microbes, including spores and cysts
			* Disinfection: process that kills many organisms, but not necessary all organisms, especially resistant forms like spores
			** High level disinfection: complete elimination of all organisms on a device, except for small numbers of bacterial spores
			* 1% sodium hypochlorite for spot cleaning
			* 10% sodium hypochlorite for disinfecting spills
			* 2% glutaraldehyde is high level disinfectant

			Sterilization

			* Dry heat sterilization using only heat (oxidation kills organisms)
			* Useful for oils, powders, and solid materials
			* 160C for 60min or 170C for 30min
			* Spore test using ''Bacillus subtilus''

			* Moist Heat autoclave (coagulate and denature proteins)
			** 121C @ 15 psi for 15-30min
			** Useful for wide variety of materials, including liquids (won't evaporate)
			*** Avoid oils and powders (repel moisture)
			*** Blood and serum will lyse/coagulate
			*** Spore test using ''Geobacillus stearothermophilus''
			* UV Sterilization
			** 265nm to damage DNA
			** Does not penetrate
			* Ionizing radiation
			** Cobalt-60
			* Gas Sterilization
			** Ethylene oxide (alkylating agent - blocks metabolism)
			** Requires longer exposure (1-6 hours)
			** Useful for larger or sensitize items

			Sterilization Controls

			* Autoclave: ''Geobacillus stearothermophilus''
			* ETO and dry heat: ''Bacillus subtilis''
			* Spore strips: clear broth = inactivated, turbid broth = growth
			* Chemical indicators (e.g., autoclave tape) that changes colour if correct temperature is reached

			Air Filtration

			* HEPA filters 0.3μm
			* Class I BSC
			** Protect only the worker and environment (not items within BSC)
			** Draw in room air through front opening
			* Class II BSC (Risk group 1-3)
			** Protects worker, environment, and items within BSC
			** Draws air through front sash into grill and filter
			* Class III BSC (Risk group 1-4)
			** Protects worker, environment, and items within BSC
			** Inside of BSC is completed enclosed
			*** Use attached gloves to handle items
			** Air is filtered twice

Admin: Created page with "Biological Safety * Universal precautions: first guidelines, centered around blood-borne pathogen precautions * Body substance isolation (BSI): updated guidelines for protection against all bodily fluids that may potentially transmit disease, as well as additional protection against certain diseases (e.g., airborne disease) * Routine practises and additional precautions (standard precautions): ** Assumption that all patients may possibly be infectious even without sympt..."

2025-02-20T22:45:29Z

Created page with "Biological Safety * Universal precautions: first guidelines, centered around blood-borne pathogen precautions * Body substance isolation (BSI): updated guidelines for protection against all bodily fluids that may potentially transmit disease, as well as additional protection against certain diseases (e.g., airborne disease) * Routine practises and additional precautions (standard precautions): ** Assumption that all patients may possibly be infectious even without sympt..."

New page

Biological Safety

* Universal precautions: first guidelines, centered around blood-borne pathogen precautions
* Body substance isolation (BSI): updated guidelines for protection against all bodily fluids that may potentially transmit disease, as well as additional protection against certain diseases (e.g., airborne disease)
* Routine practises and additional precautions (standard precautions):
** Assumption that all patients may possibly be infectious even without symptoms
** Routine use of PPE and practises (e.g., handwashing) to prevent the spread of disease
** If there are specific infection control concerns, then additional precautions may be used
*** Contact
*** Droplet
*** Airborne

Safety - Revision history

Admin at 23:10, 20 February 2025