Admin at 15:41, 13 February 2025

2025-02-13T15:41:41Z

← Older revision		Revision as of 11:41, 13 February 2025
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	*** Extra/missing reactions: see below		*** Extra/missing reactions: see below
	* Perform gel screening		* Perform gel screening

			==== Rh Control ====

			* Rh Control used when:
			** One reagent reacts but the other doesn't
			** IAT testing is needed for Weak D testing
			** Patient is AB Rh Positive (all forward grouping is positive - use control to check for agglutination)
			*** Rh Control should be negative
			* Causes of positive Rh control

			==== Weak D Testing ====
			Detect weaker expression of D antigen using IAT method

			* Perform routine Anti-D1 and Anti-D2 (may cause sensitization)
			** Grade IS results
			* Incubate both tubes 37°C, wash tubes (to remove unbound antibodies), and add AHG
			* If sensitization occurs, AHG will cause agglutination
			* Check Rh control is negative to verify validity of test
			** If positive, could be due to in vivo coating of cells with antibody (e.g., auto-antibodies, mother's antibody on fetal cells, antibody on donor cells, etc.)
			* Confirm negatives by adding Coomb's Control Cells

			__NOEDITSECTION__

Admin: Created page with "This provides a general overview of the transfusion type and screen as well as further investigations and crossmatching workflow. ==== Specimen Integrity Checks ==== Checks for possibly discrepancies or issues affecting specimen quality and patient safety * Correct number of tubes should be accounted for * Labels must be fully visible without any cut-off information * Name and MRN must match requisition * Tubes must be initialed and dated by collector; this must match..."

2024-10-24T01:26:59Z

Created page with "This provides a general overview of the transfusion type and screen as well as further investigations and crossmatching workflow. ==== Specimen Integrity Checks ==== Checks for possibly discrepancies or issues affecting specimen quality and patient safety * Correct number of tubes should be accounted for * Labels must be fully visible without any cut-off information * Name and MRN must match requisition * Tubes must be initialed and dated by collector; this must match..."

New page

This provides a general overview of the transfusion type and screen as well as further investigations and crossmatching workflow.

==== Specimen Integrity Checks ====
Checks for possibly discrepancies or issues affecting specimen quality and patient safety

* Correct number of tubes should be accounted for
* Labels must be fully visible without any cut-off information
* Name and MRN must match requisition
* Tubes must be initialed and dated by collector; this must match the requisition
* Sample must be <24h old
* Tube must be collected properly (adequate volume, correct tube type [EDTA])
** Must not be clotted or grossly hemolyzed

==== Type & Screen ====

* Perform IS forward and reverse typing
** Anti-A, Anti-B, Anti-D1, Anti-D2, Rh Control, A1 cell, B cell
** Record reactions, reactions are expected to be 2+ or greater
** Check for discrepancies and mixed fields
*** Discrepancies may include extra or missing reverse reactions
*** Mixed fields may appear as some grade of agglutination alongside a turbid/unclear background. Check microscopically to confirm (will see two populations, e.g., agglutinated cells and unagglutinated cells)
** If there are discrepancies, attempt to correct them
*** Mixed field: check transfusion history in past 3 months that explains results
*** Extra/missing reactions: see below
* Perform gel screening

Transfusion Workflow - Revision history

Admin at 15:41, 13 February 2025