Hematoxylin & Eosin Staining: Difference between revisions
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Revision as of 12:12, 20 February 2024
Troubleshooting
Preventative Measures
Check reagents before use:
- Hematoxylin should appear dark burgundy-purple with no sheen
- If metallic sheen or brown/red-brown appearance, may be over-oxidized and needs replacement
- Filter all coloured reagents to remove precipitate