Immunoassays
Types of Immunoassays
- Enzyme-linked immunosorbent assay (ELISA)
- Enzyme multiplied immunoassayu technique (EMIT)
- Enzyme immunoassay (EIA)
- Radioimmunoassay (RIA)
- Immunoradiometric assay (IRMA)
- Microparticle enzyme immunoassay (MEIA)
- Radi
- FIA
| Assay | Labelled Molecule | Competitive or Non-competitive | Homogeneous or Heterogenous | Advantages | Disadvantages |
|---|---|---|---|---|---|
| ELISA (Direct) | Ab | Non-competitive | Heterogenous | ||
| ELISA (Indirect) | Ag | Competitive | Heterogenous? | ||
| EIA | |||||
| EMIT | Homogenous | ||||
| RIA | Ag | Competitive | Heterogenous | ☢ | |
| IRMA | Ab | Non-competitive | Heterogenous | ☢ | |
| MEIA |
Separation Methods
- Precipitation
- Chemical
- Immunological
- Phase adsoprtion
- Liquid-phase adsoroption
- Solid-phase adsorption
- Differential migration
- Column separation (ion exchange by charge, gel filtration via size exclusion)
- Centrifugation
- Electrophoresis