Specimen Types:
| Specimen Type
|
Uses
|
Collection Info
|
Interferences/Rejection Criteria
|
| EDTA (lavender top)
|
- CBC, differential, blood morphology
|
- Transport within 1h
- Run within 24h
|
|
| Sodium citrate (light blue top)
|
- Coagulation
- Citrated platelet count (if EDTA-induced platelet clumping occurs)
- WBC and PLT count (multiply by x1.1 to correct for dilution)
- MPV reported
- Depending on site, other parameters can also be reported from citrated tube (x1.1)
|
- Run within 4h
- Spin to separate plasma and freeze long term
- 9:1 fill ratio
|
- Improper fill (over- or under-filled)
- Overfill -> clumping (not enough anticoagulant)
- Underfill -> prolonged coag results (too much anticoagulant)
- Improper mixing
- High hematocrit (requires adjusted hematocrit)
- Clotted
- Gross hemolysis
|
|
|
|
|
|
Adjusted citrate for hematocrit
- Adjust whenever ≥0.55
- C= (1.85 x 10^3) (100-HCT) (V)
- C = volume of sodium citrate that should remain
- HCT = hematocrit
- V = blood volume (e.g., 2.7 mL sodium citrate tube)
